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By John R. W. Masters

This new version of Animal phone tradition covers new or up-to-date chapters on telephone authentication, serum-free tradition, apoptosis assays, FISH, genetic amendment, scale-up, stem mobilephone assays, third-dimensional tradition, tissue engineering and cytotoxicity assays. distinct protocols for a large choice of tools give you the middle of every bankruptcy, making new method simply available. all people operating in organic and scientific study, no matter if in academia or a advertisement association, practicing cellphone tradition will gain enormously from this e-book.

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Animal Cell Culture: A Practical Approach

This new version of Animal phone tradition covers new or up to date chapters on mobilephone authentication, serum-free tradition, apoptosis assays, FISH, genetic amendment, scale-up, stem cellphone assays, three-dimensional tradition, tissue engineering and cytotoxicity assays. distinct protocols for a wide selection of equipment give you the middle of every bankruptcy, making new method simply available.

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J. Cell Biol, 96, 1172. 18. Sattler, C. , Sattler, G. L, and Pitot, H. C. (1978). , 38, 1539. 19. Freshney, R. I. (1992). Culture of epithelial cells. Wiley-Liss, New York. 20. , et al. (1998). J. Cell Physiol, 174, 99. 21. Pereira-Smith, O. M. and Smith, J. (1988). Proc. Natl Acad. Sri. USA, 85, 6042. 22. , Barrett, J. , and Oshimura, M. (1994). , 54, 6090. 23. Bodnar, A. , Holt, S. , Morin, G. , et al. (1998). Science, 279, 349. 24. Grander, D. (1998). Med. Oncol, 15, 20. 25. Freshney, R. I. and Freshney, M.

Although cells can presumably attach by electrostatic forces alone, it has been found that the mechanism of attachment is similar, whatever the substrate charge (14). The important factor is the net negative charge, and surfaces such as glass and metal which have high surface energies are very suitable for cell attachment. g. g. high voltage discharge, UV light, high energy electron bombardment). One or more of these methods are used by manufacturers of tissue culture grade plastics. The result is to increase the net negative charge of the surface (for example by forming negative carboxyl groups) for electrostatic attachment.

The largest stationary flask routinely used in laboratories is the Roux bottle (or disposable plastic equivalent) which gives a surface area for cell attachment of 175-200 cm2 (depending upon type), needs 100-150 ml medium, and utilizes 750-1000 cm2 of storage space. This vessel will yield 2 x 107 diploid cells and up to 108 heteroploid cells. e. manipulations have to be repeated 100 times). In addition the cubic capacity of incubator space needed is over 100 litres. Clearly there comes a time in the scale-up of cell production when one has to use a more efficient culture system.

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